Objective: To interface with native networks to extract information on cell state and identity and to predictable actuate changes in transgene expression to identify, select, and control cell fates.

Why? Over the last decade, advances in genome engineering and stem cell biology have massively expanded the potential of engineered cells as therapeutics. To rapidly advance gene and cell-based therapies, synthetic biology aims to harness the power of native biology via gene circuits capable of dynamically prescribing cellular processes, states, and identities.

Challenges. Predictive performance and design as well as translatable tools and techniques remain limited for primary cells. Using primary cells as a testbed, we are developing novel tools for identifying, selecting, and controlling primary cells with gene circuits.

Vision. Using a combination of molecular engineering and computational modeling, we are improving the ability to predict circuit performance and to detect cell state and identity. These tools are accelerating the speed at which we can engineer cells and extract biological insights.